Contact Information 
Address(Office)E12-3030Zheng Jun
(Lab)N22-3007
Phone(Office)8822 4509
(Lab)8822 4132
Fax8822 2314
EmailJunZheng@um.edu.mo
Education
Ph.D.Department of Biological Sciences, National University of Singapore (2007)
M.Sc.Institute of Genetics and Cytology, Northeast Normal University, Changchun, China (2002)
B.Sc.School of Life Science, Northeast Normal University, Changchun, China (1999)
Positions
2015-presentAssistant Professor, Faculty of Health Sciences, University of Macau
2013.4-2014.12Senior Scientist /Team Leader, Vela Diagnostics, Singapore
2012.1-2013.3Scientist, Vela Diagnostics, Singapore
2009.9-2011.12Research Fellow, Novartis Institute for Tropical Diseases, Singapore
2008.3-2009.8Research Fellow, Dept. of Microbiology and Molecular Genetics, Harvard Medical School, USA, Advisor: Dr. John Mekalanos
Research Interests
We are interested to understand how bacterial pathogens infect hosts as well as how do they get tolerance to antibiotic killing, aiming to find solutions for global crisis of the antibiotic resistance.

  • Bacterial pathogenesis
    We attempt to understand the molecular mechanism that bacterial pathogens use to attack and exploit hosts. Currently we mainly focus on type VI secretion system (T6SS), a complex molecular nanomachine for translocation of effector proteins to eukaryotic cells or prokaryotic competitors. With Vibrio parahaemolyticus as a model, we are dedicating to the role of T6SSs during bacterial survival and infection as well as the underlying mechanism of action of the effectors.
  • Mechanism of bacterial death
    Antibiotics resistance has become a global crisis due to escalating evolution of resistance coupled with a diminished antibiotic pipeline. It is highly urgent for actions to be taken to develop new antibiotics that can fight the widely-distributed drug resistant bacteria. One efficient way to achieve this goal is to understand how the current antibiotics act on bacteria and how bacteria respond to antibiotic killing as well as how bacteria develop antibiotic resistance. Such effort could lead to the identification of novel candidates as a target for new chemical identities with novel mode of action. With Acinetobacter baumannii and Escherichia coli as the model microorganisms, we employ a diverse toolkit, including genetics, biochemical, chemical genetic and metabolomic profiling, for key factors leading to bacterial tolerance to antibiotic killing. Our goal is to identify new targets for the development of more effective chemotherapies.
  • Development of new antibacterials
    We look for new antibacterial reagent for resistant bacterial pathogens and are interested in multi-discipline collaborations.
Representative Publications

#Co-first author; * Corresponding author

  1. Ji, X., J. Zou, H. Peng, A.S. Stolle, R. Xie, H. Zhang, B. Peng, J.J. Mekalanos* & J. Zheng*. Alarmone Ap4A is elevated by aminoglycoside antibiotics and enhances their bactericidal activity.Proc Natl Acad Sci U S A. doi: 10.1073/pnas.1822026116. (2019).
  2. Zhang, J.Y., F. Faucher, W. Zhang, S. Wang, M. Wells, K. Poole, J.Zheng & Z. Jia. Structure-guided disruption of the pseudopilus tip complex inhibits the Type II secretion in Pseudomonas aeruginosa. PLoS Pathogens 14:e1007343 (2018).
  3. Zou, J., W. Zhang, H. Zhang, X. D. Zhang, B. Peng, & J. Zheng*. Studies on Aminoglycoside Susceptibility Identify a Novel Function of KsgA to Secure Protein Translational Fidelity during Antibiotic Stress. Antimicrob. Agents. Ch. 62(10). pii: e00853-18 (2018).
  4. Xie, R., X.D.Zhang, Q. Zhao, B. Peng&J. Zheng*.Analysis of Global Prevalence of Antibiotic Resistance in Acinetobacter baumanniiInfections Disclosed a Faster Increase in OECD Countries. Emerg. Microbes. Infect.7:31(2018).
  5. Wang,S., Z. Li, Y. Liu, G. Feng, J. Zheng*, Z. Yuan*, & X. Zhang*. Activatable Photoacoustic and Fluorescent Probe of Nitric Oxide for Cellular and in Vivo Imaging. Sensor & Actuator B. 267: 403-411(2018).
  6. Ye, J.Z., X. M. Lin, Z. X. Cheng, Y. B. Su, W. X. Li, F. M. Ali, J. Zheng & B. Peng. Identification and efficacy of glycine, serine and threonine metabolism in potentiating kanamycin-mediated killing of Edwardsiella piscicida.J. Proteomics 183: 34-44 (2018)
  7. Ye, J., Y.B. Su, X. Lin, S.S. Lai, W. Li, F. Ali, J. Zheng & B. Peng. Alanine enhances aminoglycosides-induced ROS production by proteomic analysis. Front. Microbiol. 9:29 (2018).
  8. Gao, D. X. Ji, J. Wang, Y. Wang, D. Li, Y. Liu, K. Chang, J. Qu, J. Zheng* & Z. Yuan*. Engineering Protein-Based Nanoplatform as Antibacterial Agents for Light Activated Dual-Modal Photothermal and Photodynamic Therapy of Infection in Both the NIR I and II Windows(Accepted).J. Mater. Chem. B 6: 732-739 (2018).
  9. Kuok, K.I., P.C. Ng, X. Ji, C. Wang, W.W. Yew, D.P.C. Chan, J. Zheng, S.M. Lee & Wang R. Supramolecular strategy for reducing the cardiotoxicity of bedaquiline without compromising its antimycobacterial efficacy. Food. Chem. Toxicol.119:425-429.(2018)
  10. Li. S. #, N. Jiang#, W. Zhao, Y. F. Ding, Y. Zheng, L. H. Wang, J. Zheng* & Wang R*. An eco-friendly in situ activatable antibiotic via cucurbit[8]uril-mediated supramolecular crosslinking of branched polyethylenimine. ChemCommun (Camb). 53: 5870-587 (2017)
  11. Li, S., J. W. Chan, Y. Li, D. Bardelang, J.Zheng, M. Y. Lee, & R. Wang. The encapsulation of clofazimine by macrocycle cucurbit[7]uril reduced its cardiotoxicity without affecting the antimycobacterial efficacy. Org.Biomol. Chem. 14: 7563-7569(2016).
  12. Moradigaravand, D.#, L.Grandjean#, E. Martinez#, H. Li #,J.Zheng #,J. Corone, D. Moore, M. E. Török, E, V. Sintchenko, H. Huang, B. Javid, J. Parkhill, J.S. Peacock & C. U. Köser. DfrA-thyA double deletion in para-aminosalicylic acid resistant Mycobacterium tuberculosis Beijing strains. Antimicrob. Agents. Ch. 60: 3864-3867 (2016).
  13. Zhang, M#, X. Kong#, J. Zheng, J. B. Wan, Y. Wang, Y. Hu, & R. Shao. Research and development of antibiotics: insights from patents and citation network. Expert Opin. Ther. Pat. 26: 617-627 (2016).
  14. Zheng, J.*, E.J.Rubin, P. Bifani, V. Mathys, V. Lim, M. Au, J. Jang, J. Nam, Dick, T., K. J. R. Walker, K. Pethe & L. R. Camacho*. Para-aminosalicylic acid (PAS) is a prodrug targeting dihydrofolate reductase in Mycobacterium tuberculosis. J. Biol. Chem.288:23447-23456 (2013).
    Recommended by F1000Prime:https://f1000.com/prime/718021399
  15. Mak, P. A. Rao P.S., Tan M.P., Lin, X., Chyba, J., Tay, J., Tan, B. H., Bifani, P., Ng, S. H., Lim, V., Lee, B. H., Cherian, J., Duraiswamy, J., Ma, J., Beer, D., Thayalan, P., Kuhe, K., Chatterjee, A., Supek, F., Wang, H., Glynne, R., Zheng, J., Boshoff, B., Barry 3rd, C., Dick, T., K. Pethe, and L. Camacho. A high throughput screen to identify inhibitors of ATP homeostasis in non-replicating Mycobacterium tuberculosis. ACS Chem Biol.7: 1190–1197 (2012).
  16. Zheng, J., B. Ho & J. J. Mekalanos. Genetic analysis of anti-amoebae and anti-bacterial activities of the type VI secretion system in Vibrio cholera. PLoS One 6: e23876 (2011).
  17. Schmitt, E. K., M. Riwanto, V. Sambandamurthy, S. Roggo, C. Miault, P. Krastel, C. Noble, D. Beer, S. Rao, M. Au, P. Niyonmattanakit, J. Zheng, D. Jeffery, K. Pethe, &. L. Camacho. The natural product cyclomarin kills Mycobacterium tuberculosis by targeting the ClpC1 subunit of the caseinolytic protease. Angew. Chem. Int. Edi.50:5889-5891 (2011).
  18. Zheng, J., O. S. Shin, D. E. Cameron & J. J. Mekalanos. Quorum sensing and a global regulator TsrA control expression of Type VI secretion and virulence in Vibrio cholerae. P. Natl. Acad. Sci. USA 107: 21128-21133 (2010).
    Recommended by F1000Prime: https://f1000.com/prime/7488957
  19. Xie, H, H.B. Yu, J. Zheng, J. P. Nie, L. J. Foster, Y.K. Mok, B. B. Finlay & K. Y. Leung. Edwardsiellatarda type III effector EseG triggers host microtubule destabilization. Infect. Immun. 78: 5011-5021(2010).
  20. Jobichen C., C. Chakraborty, M. Li, J. Zheng, L. Joseph, Y. K. Mok. K.Y., Leung & J. Sivaraman. Structural basis for the secretion of EvpC: a key type VI secretion system protein from Edwardsiellatarda. PLoS One5: e12910 (2010).
  21. Zheng, J. & K.Y.Leung. Dissection of a type VI secretion system (T6SS) in Edwardsiellatarda. Mol. Microbiol.66: 1192-1206 (2007).
  22. Zheng, J., N. Li, Y. P. Tan, J. Sivaraman, Y. K. Mok & K. Y. Leung. (2007) EscC is a chaperone for the Edwardsiellatarda T3SS putative translocon components EseB and EseD. Microbiology-UK 153: 1953-1962.
  23. Zheng, J., S. L. Tung & K. Y. Leung.Regulation of a type III and a putative secretion system (EVP) of Edwardsiellatarda by EsrC is under the control of a two-component system EsrA-EsrB.Infect. Immun. 73:4127-4137 (2005).
  24. Tan, Y. P., J. Zheng, S. L. Tung, I. Rosenshine & K. Y. Leung. Role of the type III secretion system in Edwardsiellatarda virulence.Microbiology-UK151:2301-2313(2005).
Awards
The best paper published by Journal of Biological Chemistry in the field of Microbiology in 2013
Grants
  1. PI, MOP 150,000. Start-up Research Grant (SRG2015-00006-FHS). 12/02/2015 to 11/02/2017
  2. PI, MOP 2,293,000. The Science and Technology Development Fund of Macau SAR (FDCT 066/2015/A2). 01/04/2016 to 31/03/2019
  3. PI, MOP 1,188,000. Multi-Year Research Grant (MYRG2016-00199-FHS). 01/09/2016 to 31/08/2019
  4. PI, MOP 1,490,000. Multi-Year Research Grant (MYRG2016-00073-FHS). 01/09/2016 to 31/08/2019
  5. PI, MOP 2,836,000. The Science and Technology Development Fund of Macau SAR (FDCT 058/2018/A2). Granted.
Team members:
Dr. Jun Zheng, Principle Investigator

Xia Ji, PhD candidate
Jin Zou, PhD candidate
Zhi Li, PhD candidate
Ruiqiang Xie, PhD candidate
Wenwen Zhang, PhD candidate
Jincan Luo, PhD candidate
Chang Lu, Graduate student

Former lab-members
Haibo Peng, Research Assistant
Xiuying Zhang, Research Assistant
Kosuke Obayashi, exchange student from Tokyo University of Science
Rixin Wang, Summer program student

Highly motivated researchers are always welcome to join our team. Please directly contact the PI.