| Talk title | Genome-wide characterization of circRNAs and beyond |
| Speaker | Prof. Li YANG Principal Investigator and Professor CAS-MPB Partner Institute of Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai |
| Date & Time | 25 October 2018 (Thursday) 9:30-10:30 |
| Venue | Room G003, E12 Building (University of Macau) |
| Abstract | Most of mRNAs and well-characterized long noncoding RNAs are shaped with 5′ cap and 3′ poly(A) tail. Thereby, conventional transcriptome analysis typically involved the enrichment of poly(A)+ RNAs by oligo(dT) selection. However, accumulated lines of evidence suggest that there are many RNA transcripts processed in alternative ways, which largely failed to be detected by oligo(dT) purification. By taking advantage of enrichment of non-polyadenylated (poly(A)−/ribo−) RNAs with high-throughput sequencing technologies, we have identified different types of non-polyadenylated RNAs in cells, especially covalently closed circular RNAs (circRNAs) from pre-RNA back-splicing (Yang et al, Genome Biol 2011; Zhang et al, Cell 2014; Zhang et al, Genome Res 2016; Li et al, Mol Cell 2017). Here, I will report our current understanding of circRNAs on their genome-wide annotation, and underlying mechanisms and challenges for circRNA biogenesis and functions |
