Talk title | Deep Characterisation of Immune Microenvironment |
Speaker | Prof. Boon-Eng THE Qianjun ZHANG Field Applications Specialist Bio-Gene Technology Limited Fluidigm Greater Asia Pacific Cytobank USA |
Date & Time | 06 Nov 2019 (Wed) 11:00-12:00 |
Venue | Room G002, N22 Building (University of Macau) |
Abstract | Mass Cytometry (MC) uses CyTOF® technology to enable researchers to perform comprehensive profiling of cell phenotypes and signaling pathways. Using antibodies tagged with stable metal isotopes of defined mass to stain cells in suspension could routinely measure more than 50 different markers simultaneously at single cell resolution in a single tube.
Moreover, Imaging Mass Cytometry (IMC) is an expansion of mass cytometry which allows highly multiplexed imaging of up to 37 protein markers simultaneously in a single scan and single stain. Information on cellular phenotypes, functions and interactions could be spatially obtained from FFPE, frozen tissue sections or cell smear at subcellular resolution with IMC to reveal a higher order of information.
This seminar will provide the technology overview of MC and IMC with applications into Immuno-Oncology, Translational and Clinical Research. |