江家耀教授 - Faculty of Health Sciences (FHS)

haydenlam Lam Su Hang2024-10-21T12:50:10+08:002024-10-09|

聯絡信息

職稱

助理教授

辦公室

(辦公室) E12-2012

電話

(辦公室) +853 8822 9509

電郵

edwinkong@um.edu.mo

諮詢時間

星期二、四 11:00-12:00

教學

Education
PhD	The University of Hong Kong, Hong Kong, China, 2013
BSc	The University of Hong Kong, Hong Kong, China, 2009

Position
10/2024 – Present	Assistant Professor, Faculty of Health Sciences, University of Macau
08/2019 – 09/2024	Post-doctoral Fellow, Institute of Molecular Biology, Mainz, Germany
08/2015 – 07/2019	Post-doctoral Fellow, School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China
01/2014 – 07/2015	Post-doctoral Fellow, Max Planck Institute of Biochemistry, Munich, Germany

Research Interests
My laboratory uses the combination of high-throughput functional genomics and standard molecular biological approaches to study the following areas: Roles of dysregulated protein degradation in cancer Roles of ubiquitin ligase mutations in cancer Quality control of abnormal and unnecessary proteins Targeting specificity of different ubiquitin ligases and their potential use in anti-cancer PROTACs

Research Interests

My laboratory uses the combination of high-throughput functional genomics and standard molecular biological approaches to study the following areas:

Roles of dysregulated protein degradation in cancer
Roles of ubiquitin ligase mutations in cancer
Quality control of abnormal and unnecessary proteins
Targeting specificity of different ubiquitin ligases and their potential use in anti-cancer PROTACs

Representative Publications
Wong, C. M., Tang, H. M. V., Kong, K. Y. E., Wong, G. W. O., Qiu, H., Jin, D. Y., Hinnebusch, A. G. (2010). Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p. *Nucleic Acids Research, 38(7), 2217–2228. Kong, K. Y. E., Tang, H. M. V., Pan, K., Huang, Z., Lee, T. H. J., Hinnebusch, A. G., Jin, D. Y., Wong, C. M. (2014). Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing. Nucleic Acids Research, 42(1), 643–660. Tang, H. M. V., Pan, K., Kong, K. Y. E., Hu, L., Chan, L. C., Siu, K. L., Sun, H., Wong, C. M., Jin, D. Y. (2015). Loss of APD1 in Yeast Confers Hydroxyurea Sensitivity Suppressed by Yap1p Transcription Factor. Scientific Reports, 5, 7897. Deng, J. J., Kong, K. Y. E., Gao, W. W., Tang, H. M. V., Chaudhary, V., Cheng, Y., Zhou, J., Chan, C. P., Wong, D. K. H., Yuen, M. F., Jin, D. Y. (2017). Interplay between SIRT1 and hepatitis B virus X protein in the activation of viral transcription. Biochimica et Biophysica Acta – Gene Regulatory Mechanisms, 1860(4), 491–501. Kong, K. Y. E.^#, Hung, T. N. F., Man, P. H. M., Wong, T. N., Cheng, T., Jin, D. Y.^# (2019). Post-transcriptional negative feedback regulation of proteostasis through the Dis3 ribonuclease and its disruption by polyQ-expanded Huntingtin. Nucleic Acids Research, 47(19), 10040–10058. (#: co-corresponding authors) Kong, K. Y. E., Coelho, J. P. L., Feige, M. J., Khmelinskii, A. (2021). Quality control of mislocalized and orphan proteins. Experimental Cell Research, 403(2), 112617. Kong, K. Y. E.^, Fischer, B.^, Meurer, M.^, Kats, I., Li, Z., Rühle, F., Barry, J. D., Kirrmaier, D., Chevyreva, V., San Luis, B. J., Costanzo, M., Huber, W., Andrews, B. J., Boone, C., Knop, M., Khmelinskii, A. (2021). Timer-based proteomic profiling of the ubiquitin-proteasome system reveals a substrate receptor of the GID ubiquitin ligase. *Molecular Cell, 81(11), 2460–2476. (: co-first authors) Kong, K. Y. E., Reinbold, C., Knop, M., Khmelinskii, A. (2023). Building yeast libraries to dissect terminal degrons with fluorescent timers. *Methods in Enzymology, 686:297-319. Reinbold, C., Kong, K. Y. E., Kats, I., Khmelinskii, A., Knop, M. (2023). Multiplexed protein stability (MPS) profiling of terminal degrons using fluorescent timer libraries in Saccharomyces cerevisiae. Methods in Enzymology, 686:321-344. Kong, K. Y. E.^,#*, Shankar, S., Rühle, F., Khmelinskii, A.^# (2023). Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons. *Nature Communications, 14(1):8363. (: co-first authors; #: co‑corresponding authors)

Representative Publications

Wong, C. M., Tang, H. M. V., Kong, K. Y. E., Wong, G. W. O., Qiu, H., Jin, D. Y., Hinnebusch, A. G. (2010). Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p. Nucleic Acids Research, 38(7), 2217–2228.
Kong, K. Y. E., Tang, H. M. V., Pan, K., Huang, Z., Lee, T. H. J., Hinnebusch, A. G., Jin, D. Y., Wong, C. M. (2014). Cotranscriptional recruitment of yeast TRAMP complex to intronic sequences promotes optimal pre-mRNA splicing. Nucleic Acids Research, 42(1), 643–660.
Tang, H. M. V., Pan, K., Kong, K. Y. E., Hu, L., Chan, L. C., Siu, K. L., Sun, H., Wong, C. M., Jin, D. Y. (2015). Loss of APD1 in Yeast Confers Hydroxyurea Sensitivity Suppressed by Yap1p Transcription Factor. Scientific Reports, 5, 7897.
Deng, J. J., Kong, K. Y. E., Gao, W. W., Tang, H. M. V., Chaudhary, V., Cheng, Y., Zhou, J., Chan, C. P., Wong, D. K. H., Yuen, M. F., Jin, D. Y. (2017). Interplay between SIRT1 and hepatitis B virus X protein in the activation of viral transcription. Biochimica et Biophysica Acta – Gene Regulatory Mechanisms, 1860(4), 491–501.
Kong, K. Y. E.^#, Hung, T. N. F., Man, P. H. M., Wong, T. N., Cheng, T., Jin, D. Y.^# (2019). Post-transcriptional negative feedback regulation of proteostasis through the Dis3 ribonuclease and its disruption by polyQ-expanded Huntingtin. Nucleic Acids Research, 47(19), 10040–10058. (#: co-corresponding authors)
Kong, K. Y. E., Coelho, J. P. L., Feige, M. J., Khmelinskii, A. (2021). Quality control of mislocalized and orphan proteins. Experimental Cell Research, 403(2), 112617.
Kong, K. Y. E.^*, Fischer, B.^*, Meurer, M.^*, Kats, I., Li, Z., Rühle, F., Barry, J. D., Kirrmaier, D., Chevyreva, V., San Luis, B. J., Costanzo, M., Huber, W., Andrews, B. J., Boone, C., Knop, M., Khmelinskii, A. (2021). Timer-based proteomic profiling of the ubiquitin-proteasome system reveals a substrate receptor of the GID ubiquitin ligase. Molecular Cell, 81(11), 2460–2476. (*: co-first authors)
Kong, K. Y. E., Reinbold, C., Knop, M., Khmelinskii, A. (2023). Building yeast libraries to dissect terminal degrons with fluorescent timers. Methods in Enzymology, 686:297-319.
Reinbold, C., Kong, K. Y. E., Kats, I., Khmelinskii, A., Knop, M. (2023). Multiplexed protein stability (MPS) profiling of terminal degrons using fluorescent timer libraries in Saccharomyces cerevisiae. Methods in Enzymology, 686:321-344.
Kong, K. Y. E.^*,#, Shankar, S.*, Rühle, F., Khmelinskii, A.^# (2023). Orphan quality control by an SCF ubiquitin ligase directed to pervasive C-degrons. Nature Communications, 14(1):8363. (*: co-first authors; #: co‑corresponding authors)

Awards
2012	Graduate Student Travel Scholarship, Tech Dragon Limited
2011	Ming K. Jeang Scholarship, The Society of Chinese Bioscientists in America
2009	University Postgraduate Fellowship, The University of Hong Kong

Research Grants
07/2017 – 12/2019	Principal Investigator for the project “Roles of the chromatin architectural protein CTCF in hepatitis B virus transcription” funded by Health and Medical Research Fund (HMRF), The Food and Health Bureau, Hong Kong, China.

Professional Activities
Journal reviewer: Science Advances; Scientific Reports; PLOS ONE