| Talk title | Molecular Analysis of Cancer with Single-cell RNAseq |
| Speaker | Prof. John ZHONG Assistant Professor School of Dentistry, University of Southern California |
| Date & Time | 02 January 2019 (Wed) 10:00-11:00 |
| Venue | Room G002, N22 Research Building (University of Macau) |
| Abstract | With conventional gene expression profiling, information concerning cellular heterogeneity is often lost in the physical mixing and averaging of millions of cells. Single-cell transcriptome analysis has the potential to address these issues. Most cancer clinical samples are mixtures of normal and cancer cells. This heterogeneity becomes a hurdle for molecular analysis of cancer with bulk lysates which is a physical mixture of cancer and normal cells. Our laboratory uses a phase-switch droplet microfluidic platform and next generation sequencing to address this challenge. I will discuss the tools and methods we developed for molecular analysis of cancer cells. |