2020-04-23T15:58:46+08:002019-01-03|Seminars and Workshops|

Talk title Molecular Analysis of Cancer with Single-cell RNAseq
Speaker Prof. John ZHONG
Assistant Professor
School of Dentistry, University of Southern California
Date & Time 02 January 2019 (Wed)
Venue Room G002, N22 Research Building (University of Macau)
Abstract With conventional gene expression profiling, information concerning cellular heterogeneity is often lost in the physical mixing and averaging of millions of cells. Single-cell transcriptome analysis has the potential to address these issues. Most cancer clinical samples are mixtures of normal and cancer cells. This heterogeneity becomes a hurdle for molecular analysis of cancer with bulk lysates which is a physical mixture of cancer and normal cells. Our laboratory uses a phase-switch droplet microfluidic platform and next generation sequencing to address this challenge. I will discuss the tools and methods we developed for molecular analysis of cancer cells.