2020-04-22T10:07:45+08:002019-09-10|Seminars and Workshops|

Talk title  Dissecting Chromatin Structure Using Super Resolution Microscopy
Speaker Prof. Maria Pia COSMA
Senior Scientist and ICREA Research Professor
Center for Genomic Regulation, Barcelona, Spain
Date & Time 09 Sep 2019 (Mon)
Venue Room G004, E12 Building (University of Macau)
Abstract One of the interests of Prof. Maria’s group is to dissect the functional modifications of the chromatin during the process of somatic cell reprogramming to pluripotency. By using quantitative super-resolution nanoscopy, we identified a novel chromatin fiber assembly and the relation among the decoded structure and naïve pluripotency. Prof. Maria’s group showed that nucleosomes arrange in groups of various sizes along the chromatin fiber, which they named ‘nucleosome clutches’ (in analogy with clutches of eggs). Clutches are interspersed with nucleosome-free regions. We also dissected at nanoscale resolution level DNA contacts and looping in specific loci. They are inspecting the 3D-structure of the chromatin and fiber looping across the chromosomes and how the spatial organization of the chromatin fibers functionally change in stem and somatic cells.