2024-10-10T17:23:10+08:002019-11-29|
Contact Information
Title
Professor
Office
(Office) E12-4014 / (Lab) N22-4002a
Phone
(Office) +853 8822 4992 / (Lab) +853 8822 2941
Fax
+853 8822 2933
Email
ECheung@um.edu.mo
Consultation Hours

Mon, Thu 10:00 – 11:00

Teaching

HSCI3004 Genomics and Bioinformatics
HSCI8118 Advanced Genetics and Bioinformatics
HSCI8006 Advanced Molecular Cell Biology

Research Team
Name Position Office Phone Email
Ka I CHEANGResearch AssistantN22-4001+853 8822 2933eviecheang@um.edu.mo
Ching Man FOOPhD Student
Wei CAOPhD Student
Chen CHANGPhD Student
Mokan DENGPhD StudentN22-4001+853 8822 2933
Cuiting ZHANGPhD StudentN22-4001+853 8822 2933
Bo LIPhD StudentN22-4001+853 8822 2933
I WUPhD StudentN22-4001+853 8822 2933
Yifan SUPhD Student
Tin CHEONGPhD Student
Education
Postdoctoral Research Fellow Department of Molecular Biology and Genetics, Cornell University (1999-2004)
PhD Molecular Biology, School of Biological Sciences, Faculty of Science and Engineering, University of Manchester (1995-2000)
BA Biochemistry and Molecular Biology, Department of Molecular and Cell Biology, University of California, Berkeley (1988-1992)
Position
2013 – Present Professor, Faculty of Health Sciences, University of Macau
2011 – 2013 Adjunct Associate Professor, Department of Biochemistry, National
University of Singapore
2006 – 2011 Adjunct Assistant Professor, Department of Biochemistry, National
University of Singapore
2006 – 2013 Faculty, NUS Graduate School for Integrative Sciences & Engineering, National University of Singapore
2006 – 2013 Adjunct Assistant Professor, School of Biological Sciences, Nanyang Technological University
2004 – 2013 Senior Investigator, Cancer Biology and Pharmacology, Genome
Institute of Singapore
1996 – 1998 Graduate Research/Teaching Assistant, School of Biological Sciences, the University of Manchester
Research Interests
My lab is interested in understanding the molecular details of nuclear hormone signaling and how these pathways relate to disease conditions, including cancers. Currently, we are investigating how lipophilic hormones such as estrogens and androgens regulate the transcriptional activities of nuclear hormone receptors in the context of a chromatin environment. We employ a combination of powerful biological tools, including modern genetic and cell-based techniques (RNAi, ChIP, and FISH) and next-generation sequencing approaches (ChIP-Seq, RNA-Seq, and ChIA-PET), to define the molecular mechanisms of transcriptional regulation by estrogen and androgen receptors. The findings from our work will have direct impact towards the development of therapeutic targets for the wide range of diseases and disorders in which these and other nuclear receptors play a role.
Representative Publications
  • Sun, X., Hou, T., Cheung, E., Iu, T. N. T., Tam, V. W. H., Chu, I. M. T., Tsang, M. S. M., Chan, P. K. S., Lam, C. W. K., and Wong, C. K. (2019) Anti-Inflammatory Mechanisms of the Novel Cytokine Interleukin-38 in Allergic Asthma. Cell Mol Immunol 17 (6), 631-646
  • Song, C. C., Xu, F. X., Ren, Z. L., Zhang, Y. M., Meng, Y., Yang, Y. Q., Lingadahalli, S., Cheung, E., Li, G., Liu, W. W., Wan, J. B., Zhao, Y., and Chen, G. K. (2019) Elevated Exogenous Pyruvate Potentiates Mesodermal Differentiation through Metabolic Modulation and Ampk/mTOR Pathway in Human Embryonic Stem Cells. Stem Cell Rep 13 (2), 338-351
  • Verma, A., Lam, Y. M., Leung, Y. C., Hu, X., Chen, X., Cheung, E., and Tam, K. Y. (2018) Combined Use of Arginase and Dichloroacetate Exhibits Anti-Proliferative Effects in Triple Negative Breast Cancer Cells. J Pharm Pharmacol 71 (3), 306-315
  • Yan, L., Jiang, B., Li, E., Wang, X. Y., Ling, Q. J., Zheng, D. J., Park, J. W., Chen, X., Cheung, E., Du, X., Li, Y. C., Cheng, G., He, E. X., and Xu, R. H. (2018) Scalable Generation of Mesenchymal Stem Cells from Human Embryonic Stem Cells in 3D. Int J Biol Sci 14 (10), 1196-1210
  • Liu, W. W., Ren, Z. L., Lu, K., Song, C. C., Cheung, E. W., Zhou, Z., and Chen, G. K. (2018) The Suppression of Medium Acidosis Improves the Maintenance and Differentiation of Human Pluripotent Stem Cells at High Density in Defined Cell Culture Medium. Int J Biol Sci 14 (5), 485-496
  • White, M., Tsouko, E., Lin, C. C., Rajapakshe, K., Spencer, J., Wilkenfeld, S., Vakili, S., Pulliam, T., Awad, D., Nikolos, F., Katreddy, R. R., Kaipparettu, B. A., Sreekumar, A., Zhang, X. L., Cheung, E., Coarfa, C., and Frigo, D. (2018) GLUT12 Promotes Prostate Cancer Cell Growth and is Regulated by Androgens and CaMKK2 Signaling. Endocr-Relat Cancer 25 (4), 453-469
  • Deng, H. L., Zeng, J. M., Zhang, T., Gong, L. C., Zhang, H. J., Cheung, E., Jones, C., and Li, G. (2018) Histone H3.3K27M Mobilizes Multiple Cancer/Testis (CT) Antigens in Pediatric Glioma. Mol Cancer Res 16 (4), 623-633
  • *Zhang, Z., *Chng, K. R., *Lingadahalli, S., Chen, Z., Liu, M. H., Guan, P., Do H. H., Cai, S., Rinaldi, N., Poh, H. M, Li, G., Sung, Y. Y., Heng, C. L., Core, L. J., Tan, S. K., Ruan, X., Lis, J. T., Kellis, M., Ruan, Y., Sung, W. K., and Cheung, E. (2018) An AR-ERG Transcriptional Signature Defined by Long Range Chromatin Interactomes in Prostate Cancer Cells. Genome Research (Accepted)*Equal contribution.
  • Zhu, D., Zhao, X., Cui, G., Chang, S., Hu, L., See, Y. X., Lim, G. M. L., Guo, D., Chen, X., Robson, P., Luo, Y., and Cheung, E.(2018) Single-Cell Transcriptome Analysis Reveals Estrogen Signaling Coordinately Augments One-Carbon, Polyamine, and Purine Synthesis in Breast Cancer. Cell Reports 25, 2285-2298
  • Lingadahalli, S., Jadhao, S., Sung, Y. Y., Chen, M., Hu, L., Chen, X., and Cheung, E. (2018) Novel lncRNA LINC00844 Regulates Prostate Cancer Cell Migration and Invasion Through AR Signaling. Molecular Cancer Research, doi: 10.1158/1541-7786.MCR-18-0087.
  • Chen, X., Miao, Z., Divate, M., Zhao, Z., and Cheung, E.(2018) KM-Express: An Integrated Online Patient Survival and Gene Expression Analysis Tool for the Identification and Functional Characterization of Prognostic Markers in Breast and Prostate Cancers. Database doi: 10.1093/database/bay069.
  • Divate, M. and Cheung, E. (2018) GUAVA: a Graphical User interface for the Analysis and Visualization of ATAC-seq data. Frontiers in Genetics, doi: 10.3389/fgene.2018.00250.
  • Hu, J., Tan, S. K., Lim, M. G. L., Chang, S. H., Cui, G., Liu, S., Narasimhan, K., New, S. Y., Wang, X., Chen, C., Chakravarty, H., Kolatkar, P. R., Tam, K., Qian, L., Su, X., Jauch, R., and Cheung, E. (2018) Identification of a Wells-Dawson Polyoxometalate-Based AP-2g Inhibitor with Pro-Apoptotic Activity. Biochemical Journal, 475: 1955-1977.
  • *Chen, X., Yang, C., Xie, S., and *Cheung, E.(2018) Long Non-coding RNAs GAS5 and ZFAS1 are Prognostic Markers Involved in Translation Targeted by miR-940 in Prostate Cancer. Oncotarget 9:1048-1062. *Co-corresponding authors.
  • Aung, K. M., Lim, M. G., Hong, S., *Cheung, E., and *Su, X. (2017) Determination of DNA Binding Behavior of FoxA1 Constructs Using a Gold Nanoparticle-Based High Throughput Assay. Journal of Molecular Engineering and Materialsorg/10.1142/S2251237316400128 *Co-corresponding authors.
  • Blessing, A., Rajapakshe, K., ReddyBollu, L., Shi, Y., White, M., Pham, A., Lin, C. C., Jonsson, P., Cortes, C., Cheung, E., LaSpada, A., Bast, R., Merchant, F., Coarfa, C., and Frigo, D. (2017) Transcriptional Regulation of Core Autophagy and Lysosomal Genes by the Androgen Receptor Promotes Prostate Cancer Progression. Autophagy 13 (3), 506-521
  • Sun, F., Zhang, Z. W., Tan, E. M., Lim, R., Li, Y., Wang, X. C., Chua, S. E., Li, J., Cheung, E., and Yong, E. L. (2016) Icaritin Suppresses Development of Neuroendocrine Differentiation of Prostate Cancer through Inhibition of Il-6/STAT3 and Aurora kinase A Pathways in Tramp Mice. Carcinogenesis 37 (7), bgw044
  • Blessing, A., Ganesan, S., Rajapakshe, K., Ying, S. Y., Reddy Bollu, L., Shi, Y., Cheung, E., Coarfa, C., Chang, J., McDonnell, D., and Frigo, D. (2015) Identification of a Novel Coregulator, Sh3yl1, That Interacts with the Androgen Receptor N-Terminus. Mol Endocrinol 29 (10), 1426-1439
  • Ling, L., Tan, S. K., Goh, T. H., Cheung, E., Nurcombe, V., van Wijnen, A., and Cool, S. (2015) Targeting the Heparin-Binding Domain of Fibroblast Growth Factor Receptor 1 as a Potential Cancer Therapy. Mol Cancer 14 (1), 136
  • Narasimhan, K., Micoine, K., Lacôte, E., Thorimbert, S., Cheung, E., Hasenknopf, B., and Jauch, R. (2014) Exploring the Utility of Organo-Polyoxometalate Hybrids to Inhibit SOX Transcription Factors. Cell Regeneration 3 (1), 10
  • Liu, M. H. and Cheung, E.(2014) Estrogen Receptor-Mediated Long-Range Chromatin Interactions and Transcription in Breast Cancer. Molecular and Cellular Endocrinology 382, 624-632.
  • New, S. Y., Aung, K. M., Lim, M. G., Hong, S., Tan, S. K., Lu, Y., *Cheung, E., and *Su, X. (2014)
    Fast Screening of Ligand-Protein Interactions Based on Ligand-Induced Protein Stabilization of Gold Nanoparticles. Analytical Biochemistry86, 2361-70. *Co-corresponding authors.
  • Aung, K. M., New, S. Y., Hong, S., Sutarlie, L., Lim, M. G., Tan, S. K., *Cheung, E., and *Su, X. (2014) Studying Forkhead Box Protein A1-DNA Interaction and Ligand Inhibition Using Gold Nanoparticles, Electrophoretic Mobility Shift Assay, and Fluorescence Anisotropy. Analytical Biochemistry448, 95-104. *Co-corresponding authors.
  • Lukman, S., Aung, K. M., Lim, M. G., Hong, S., Tan, S. K., *Cheung, E., and *Su, X. (2014) Hybrid Assembly of DNA-Coated Gold Nanoparticles with Water Soluble Conjugated Polymers for Studying Protein-DNA Interaction and Ligand Inhibition. RSC Advances4, 8883-8893. *Co-corresponding authors.
  • Sutarlie, L., Aung, K. M., Lim, M. G., Lukman, S., *Cheung, E., and *Su, X. (2014) Studying Protein-DNA Complexes using Gold Nanoparticles by Exploiting Particle Aggregation, Refractive Index Change, and Fluorescence Quenching and Enhancement Principles.Plasmonics doi:1007/s11468-013-9655-2. *Co-corresponding authors.
  • Cheung, E.and Sun, Y. Y. (2013) Transcriptional Network of Castrate Resistant Prostate Cancer. Endocrine Related Cancer 21, R1-R11.
  • Sung, Y. Y., and Cheung, E.(2013) Antisense Now Makes Sense: Dual Modulation of Androgen-Dependent Transcription by CTBP1-AS. The EMBO Journal 12, 1653-1654.
  • , K. R., and Cheung, E.(2012) Sequencing the Transcriptional Network of AR in Prostate Cancer. Cancer Letters 340, 254-260.
  • Chng, K. R., Chang, C. W., Tan, S. K., Yang, C., Hong, S. Z., Sng, N. Y. W., and Cheung, E.(2012) A Transcriptional Repressor Co-Regulatory Network Governing Androgen Response in Prostate Cancer. The EMBO Journal 31, 2810 – 2823.
  • Tan, P. Y., Chang, C. W., Chng, K. R., Abayratna, S., Sung, W. K., and Cheung, E.(2012)
    Integration of Regulatory Networks by NKX3.1 Promotes Androgen-Dependent Prostate Cancer Survival. Molecular and Cellular Biology 32, 399-414.
    #Highlighted as a hot article in the Spotlight section of MCB
  • Li, G., Ruan, X., Auerbach, R., Sandhu, K. S., Wang, P., Orlov, Y., Poh, H. M., Goh, Y., Lim, J., Sim, H. S., Peh, S. Q., Mulawadi, F. H., Ong, C. T., Landt, T., Raha, D., Euskirchen, G., Hong, S. Z., Thoreau, H., Wei, C. L., Ge, W., Sun, Y., Fullwood, M. J., Liu, E. T., Cheung, E., Sung, W. K., Snyder, M., and Yijun, R. (2012) Extensive Chromatin Interactions Provide Architectural Basis for Transcription Regulation in Eukaryotes. Cell148, 84-98.
  • Tan, S. K., Lin, Z. H., Chang, C. W., Varang, V., Chng, K. R., Yong, E. L., Sung, W. K., and Cheung, E.(2011) AP-2 Regulates Oestrogen Receptor-Mediated Long-Range Chromatin Interaction and Gene Transcription. The EMBO Journal 30, 2569-81.
  • Zhang, Z. Z., Chang, C. W., Goh, W. L., *Sung, W. K., and *Cheung, E.(2011) CENTDIST: Discovery of Co-associated Factors by Motif Distribution. Nucleic Acid Research Suppl 2:W391-9. *Co-corresponding authors.
  • Cheung, E., and Ruan, Y. (2011) Determination of Transcription Factor Binding. Nature Genetics43,1.
  • Huang, P. Y. H., Han, Y., Handoko, L., Velkov, S., Wong, E., Cheung, E., Ruan, X., Wei, C. L., Fullwood, M. J., and Ruan, Y. (2010) Protocol: Sonication-based Circular Chromosome Conformation Capture with Next-Generation Sequencing Analysis for the Detection of Chromatin Interactions. Nature Protocol Exchangedoi:10.1038/protex.2010.207
  • Mason, C. E., Shu, F., Wang, C., Session, R. M., Kallen, R. G., Sidell, N., Yu, T., Liu, M. H., Cheung, E., Kallen, C. B. (2010) Location Analysis for the Estrogen Receptor-{alpha} Reveals Binding to Diverse ERE Sequences and Widespread Binding Within Repetitive DNA Elements. Nucleic Acid Research38, 2355-68.
  • *Joseph, R., *Orlov, Y. L., *Huss, M., Sun, W., Ukil, L., Kong, S. L., Pan, Y. F., Li, G., Lim, M., Thomsen, J. S., Ruan, Y., Clarke, N.D., Prabhakar, S., Cheung, E., and Liu, E. T. (2010) Genomic Factors Determining Binding Site Selection by Estrogen Receptor α. *Equal contributions. Molecular Systems Biology6:456
  • Fullwood, M. J., Liu, M. H., Pan, Y. F., Liu, J., Xu, H., Mohamed, Y. B., Orlov, Y. L., Velkov, S., Ho, A., Huang, P. Y. H., Chew, E. G. Y., Yao, P., Huang, H., Welboren, W-J., Han, Y., Ooi, H. S., Ariyaratne, P. N., Vega, V. B., Luo, Y., Tan, P. Y., Choy, P. Y., Abayratna, K. D. S., Zhao, B., Lim, K. S., Leow, S. C., Yow, J. S., Joseph, R., Li, H., Desai, K. V., Thomsen, J. S., Lee, Y. K., Karuturi, R. K. M., Herve, T., Bourque, G., Stunnenberg, H. G., Ruan, X., Cacheux-Rataboul, V., Sung, K. W. K., Liu, E. T., Wei, C. L., *Cheung, E., and *Ruan, Y. (2009) An Oestrogen Receptor α-Bound Human Chromatin Interactome. Nature482 (7269), 58-64. *Co-corresponding authors.
  • Pan, Y. F., Abayratna, S., Liu, M. H., Zhao, B., Hong, S. Z., Tan, P. Y., Lim, K. S., Bourque, G., Liu, E. T., and Cheung, E.(2008) Regulation of Estrogen Receptor-Mediated Long Range Transcription Via Evolutionarily Conserved Distal Response Elements. The Journal of Biological Chemistry 283, 32977-88.
  • Lin, C. Y., Vega, V. B., Thomsen, J. S., Zhang, T., Kong, S. L., Xie, M., Chiu, K. P., Lipovich, L., Barnett, D. H., Stossi, F., Yeo, A., George, J., Kuznetsov, V. A., Lee, Y. K., Charn, T. H., Palanisamy, N., Miller, L. D., Cheung, E., Katzenellenbogen, B. S., Ruan, Y., Bourque, G., Wei, C. L., and Liu, E. T. (2007) Whole-genome Cartography of Estrogen Receptor  Binding Sites. PLoS Genetics3, e87.
  • Ross, S.*, Cheung, E.*, Petrakis, T.G., Howell, M., Kraus, W. L., and Hill, C. S. (2006) Smads Orchestrate Specific Histone Modifications and Chromatin Remodeling to Activate Transcription. *Equal Contributions. TheEMBO Journal 25, 4490-4502.
  • Cheung, E., Acevedo, M. L., Cole, P. A., and Kraus, W. L.  (2005) Altered Pharmacology and Distinct Coactivator Usage for Estrogen Receptor-Dependent Transcription Through Activating Protein-1.  Proceedings of the National Academy of Sciences 102, 559-64.
  • Cheung, E., Schwabish, M. A., and Kraus, W. L.  (2003) Chromatin Exposes Intrinsic Differences in the Transcriptional Activities of Estrogen Receptors a and b.  The EMBO Journal 22, 600-11.
  • Cheung, E., Zarifyan, A. S., and Kraus, W. L.  (2002) Histone H1 Represses Estrogen Receptor Alpha Transcriptional Activity by Selectively Inhibiting Receptor-Mediated Transcription Initiation.  Molecular Cellular Biology 22, 2463-71.

*Co-corresponding authors
Research Grants
2018 – 2021 University of Macau MYRG (MOP1,500,000: PI)
2016 – 2019 FDCT Research Grant (MOP1,616,900: PI)
2015 – 2018 University of Macau MYRG (MOP2,400,000: PI)
2015 – 2018 FDCT Research Grant (MOP2,600,000: PI)
2015 – 2016 FDCT Special Equipment Fund (MOP6,750,000: PI)
2014 – 2015 University of Macau SRG (MOP150,000: PI)
2012 – 2015 A*STAR Joint Council Office Grant, Integrative Analysis of the Oncogenic Transcription Factor, AP-2gamma, in Breast Cancer Using Nanobiotechnology and Genome Biology, (SGD$1,000,000, Project Director/PI)
2011 – 2014 NMRC IRG, Novel Anti-Androgens of Botanical Origin as Multiple-Site Modulators of Androgen Receptor Signalling to Inhibit Prostate Cancer Development and Growth, (SGD$849,622, Co-PI)
2011 – 2013 A*STAR-NZ HRC JGC Grant, Oestrogen-Dependent Regulation of Gene Expression by Cohesion in Breast Cancer, (SGD$250,000, Co-PI)
2008 – 2011 A*STAR BMRC Grant, Molecular Mechanisms of Action and Human Bioaccumulation of a Novel Estrogenic Compound Secreted by a Singapore Phytoplankton Isolate, Chattonella marina, (S$604,132, Co-PI)
Patents
2013 201303835-1: A method to screen small ligands and determine protein-ligand interactions (filed)
2013 201303638-9: A hybrid sensor using metal nanoparticles and conjugated polymers (filed)
2012 13/651,141: Method of modulating a prostate cancer cell (filed)
Awards
2003 – 2004 NIH NRSA Postdoctoral Fellowship
2000 – 2003 Susan G. Komen Breast Cancer Foundation Postdoctoral Fellowship
1996 – 1998 Overseas Research Studentship Award
1995 – 1998 Frederick Craven Moore Research Studentship Award
Professional Activities
2013 – Present Editorial Board, Molecular Cancer Research
Others
Conference Presentations

  1. Cheung, E. EMBL Symposium: Cellular Heterogeneity, “Transcriptome Analysis of Androgen Signaling in Individual Prostate Cancer Cells”2015