Contact Information
Research Team
NamePositionOfficePhoneEmail
Shuhui GUOPhD StudentN22-3003+853 8822 4232
Fang WANGPhD StudentN22-3003+853 8822 4232
Chirag PARSANIAPhD StudentN22-3003+853 8822 4232
Pooja PRAKASHCHANDRA SETHIYAPhD StudentN22-3003+853 8822 4232
Liguo DONGPhD StudentN22-3003+853 8822 4232
Zhengqiang MIAOPhD StudentN22-3003+853 8822 4232
Zhiqiang DONGPhD StudentN22-3004+853 8822 4131
Qing LANPhD StudentN22-3004+853 8822 4131
Yimin ZHENGPhD StudentN22-3004+853 8822 4131
Education
PhDDepartment of Genetics, The University of Melbourne, Australia (2007)
BSc (1st Class Hons)Department of Genetics, The University of Melbourne, Australia (2002)
BScFaculty of Science, The University of Melbourne, Australia (2001)
DiplomaBiotechnology, Ngee Ann Polytechnic, Singapore (1999)
Positions
2020-presentAssociate Professor, Faculty of Health Sciences, University of Macau
2013-2020Assistant Professor, Faculty of Health Sciences, University of Macau
2008-2013Postdoctoral Research Fellow, Department of Biochemistry and Molecular Pharmacology, Harvard Medical School, USA
2007-2008Postdoctoral Research Fellow, Department of Genetics, The University of Melbourne, Australia
Research Interests
My research focuses on two broad aims:

  • To understand how pathogenic fungi cause diseases in humans.
  • To understand how transcription is regulated.

Why study Fungal Pathogenesis?

Fungal pathogens are ubiquitous in the environment, but they usually do not post serious threats to people with a healthy immune system. However, fungal pathogens can cause life-threatening diseases in individuals with a compromised immune system. In recent years, the number of immuno-compromised individuals is escalating as chemotherapy, bone marrow-transplant and organ-transplant therapies are becoming common medical procedures. Prevention and treatment of fungal infections has become a major clinical challenge. The situation is exacerbated by the lack of effective anti-fungal therapies with fatality rates of certain fungal infections being as high as 100%. Therefore, understanding fungal pathogenesis has immense medical implications and will provide valuable information towards development of new effective and life-saving anti-fungal treatments.

Why study Transcription Regulation?

Transcription is an essential molecular process whereby stored biological information is retrieved from the genome of living organisms. Proper and precise control of transcription is of fundamental importance to biology. Defects in this process are linked to many human diseases including cancers. The molecular mechanisms of transcriptional regulation in eukaryotic organisms are remarkably conserved from humans to fungi. In fact, much of our current knowledge of transcription regulation has come from fungal studies. Through studying transcription regulation in fungi, my research aims to provide important new information that is directly relevant to transcription regulatory mechanisms in humans.

Representative Publications
Journal Publication

  1. Sethiya, P., Rai, M. N., Rai, R., Parsania, C., Tan, K., and Wong, K. H. (2020) Transcriptomic Analysis Reveals Global and Temporal Transcription Changes During Candida Glabrata Adaptation to an Oxidative Environment. Fungal Biol 124 (5), 427-439
  2. Ries, L. N. A., Pardeshi, L., Dong, Z., Tan, K., Steenwyk, J. L., Colabardini, A. C., Ferreira Filho, J. A., de Castro, P. A., Silva, L. P., Preite, N. W., Almeida, F., de Assis, L. J., Dos Santos, R. A. C., Bowyer, P., Bromley, M., Owens, R. A., Doyle, S., Demasi, M., Hernandez, D. C. R., Netto, L. E. S., Pupo, M. T., Rokas, A., Loures, F. V., Wong, K. H.*, and Goldman, G. H. (2020) The Aspergillus Fumigatus Transcription Factor RglT is Important for Gliotoxin Biosynthesis and Self-Protection, and Virulence. Plos Pathog 16 (7), e1008645 (* Co-corresponding author)
  3. Zhou, P., Chan, B. K. C., Wan, Y. K., Yuen, C. T. L., Choi, G. C. G., Li, X., Tong, C. S. W., Zhong, S. S. W., Sun, J., Bao, Y., Mak, S. Y. L., Chow, M. Z. Y., Khaw, J. V., Leung, S. Y., Zheng, Z., Cheung, L. W. T., Tan, K., Wong, K. H., Chan, H. Y. E., and Wong, A. S. L. (2020) A Three-Way Combinatorial CRISPR Screen for Analyzing Interactions among Druggable Targets. Cell Rep 32 (6), 108020
  4. Valero, C., Colabardini, A. C., Chiaratto, J., Pardeshi, L., de Castro, P. A., Ferreira Filho, J. A., Silva, L. P., Rocha, M. C., Malavazi, I., Costa, J. H., Fill, T., Barros, M. H., Wong, S. S. W., Aimanianda, V., Wong, K. H., and Goldman, G. H. (2020) Aspergillus Fumigatus Transcription Factors Involved in the Caspofungin Paradoxical Effect. Mbio 11 (3), e00816-00820
  5. Yan, J. L., Bhadra, P., Li, A., Sethiya, P., Qin, L. G., Tai, H. K., Wong, K. H., and Siu, S. W. I. (2020) Deep-AmPEP30: Improve Short Antimicrobial Peptides Prediction with Deep Learning. Mol Ther Nucleic Acids 20, 882-894
  6. Vassiliadis, D., Wong, K. H., Blinco, J., Dumsday, G., Andrianopoulos, A., and Monahan, B. (2020) Adaptation to Industrial Stressors through Genomic and Transcriptional Plasticity in a Bioethanol Producing Fission Yeast Isolate. G3 (Bethesda) 10 (4), 1375-1391
  7. Qin, L. G., Li, A., Tan, K., Guo, S. h., Chen, Y. Y., Wang, F., and Wong, K. H. (2019) Universal Plasmids to Facilitate Gene Deletion and Gene Tagging in Filamentous Fungi. Fungal Genet Biol 125, 28-35
  8. Tan, K., and Wong, K. H. (2019) RNA Polymerase II ChIP-Seq-a Powerful and Highly Affordable Method for Studying Fungal Genomics and Physiology. Biophysical Reviews 11 (1), 79-82
  9. Petrenko, N., Jin, Y., Dong, L. G., Wong, K. H.*, and Struhl, K*. (2019) Requirements for RNA Polymerase II Preinitiation Complex Formation in vivo. eLife 8, e43654 (* Corresponding author)
  10. Choi, G. C. G., Zhou, P., Yuen, C. T. L., Chan, B. K. C., Xu, F., Bao, S., Chu, H. Y., Thean, D., Tan, K., Wong, K. H., Zheng, Z. L., and Wong, A. S. L. (2019) Combinatorial Mutagenesis En Masse Optimizes the Genome Editing Activities of SpCas9. Nat Methods 16 (8), 722-730
  11. Vassiliadis, D., Wong, K. H., Andrianopoulos, A., and Monahan, B. J. (2019) A Genome-Wide Analysis of Carbon Catabolite Repression in Schizosaccharomyces Pombe. Bmc Genomics 20 (1), 251
  12. Kim, S. H., Iyer, K. R., Pardeshi, L., Munoz, J. F., Robbins, N., Cuomo, C. A., Wong, K. H., and Cowen, L. E. (2019) Genetic Analysis of Candida Auris Implicates Hsp90 in Morphogenesis and Azole Tolerance and Cdr1 in Azole Resistance. mBio 10 (1), e02529-02518
  13. Veri, A., Miao, Z. Q., Shapiro, R., Tebbji, F., O’Meara, T., Kim, S. H., Colazo, J., Tan, K., Vyas, V., Whiteway, M., Robbins, N., Wong, K. H., and Cowen, L. (2018) Tuning Hsf1 Levels Drives Distinct Fungal Morphogenetic Programs with Depletion Impairing Hsp90 Function and Overexpression Expanding the Target Space. Plos Genet 14 (3), e1007270
  14. Xie, J. L., Qin, L. G., Miao, Z. Q., Grys, B., Diaz, J. D. L. C., Ting, K., Krieger, J., Tong, J. F., Tan, K., Leach, M., Ketela, T., Moran, M., Krysan, D., Boone, C., Andrews, B., Selmecki, A., Wong, K. H., Robbins, N., and Cowen, L. (2017) The Candida Albicans Transcription Factor Cas5 Couples Stress Responses, Drug Resistance and Cell Cycle Regulation. Nat Commun 8 (1), 499
  15. Petrenko, N.*, Jin, Y.*, Wong, K. H.*, and Struhl, K. (2017) Evidence That Mediator Is Essential for Pol II Transcription, but Is Not a Required Component of the Preinitiation Complex in vivo. eLife 6, e28447 (* Equal contribution)
  16. Szot, P., Yang, A., Wang, X., Parsania, C., Röhm, U., Wong, K. H., and Ho, J. (2017) PBrowse: A Web-Based Platform for Real-Time Collaborative Exploration of Genomic Data. Nucleic Acids Res 45 (9), e67
  17. Petrenko, N.*, Jin, Y.*, Wong, K. H.*#, and Struhl, K.# (2016) Mediator Undergoes a Compositional Change During Transcriptional Activation. Mol Cell 64 (3), 443-454 (* Equal contribution; # Corresponding author)
    Featured in: Malik, S., and Roeder, G.R. (2016) Mediator: A Drawbridge across the Enhancer-Promoter Divide. Molecular Cell 64(3), 433-434
  18. Leach, M., Farrer, R., Tan, K., Miao, Z. Q., Walker, L., Cuomo, C., Wheeler, R., Brown, A., Wong, K. H., and Cowen, L. (2016) Hsf1 and Hsp90 Orchestrate Temperature-Dependent Global Transcriptional Remodelling and Chromatin Architecture in Candida Albicans. Nat Commun 7, 11704
  19. Wong, K. H.*, Jin, Y.*, and Struhl, K. (2014) TFIIH Phosphorylation of the Pol II CTD Stimulates Mediator Dissociation from the Preinitiation Complex and Promoter Escape. Mol Cell 54 (4), 601-612 (* Equal contribution)
  20. Wong, K. H.*, Jin, Y., and Moqtaderi, Z. (2013) Multiplex Illumina Sequencing Using DNA Barcoding. Curr Protoc Mol Biol Chapter 7, Unit 7 11 (* Corresponding author)
  21. Suzuki, Y., Murray, S. L., Wong, K. H., Davis, M. A., and Hynes, M. J. (2012) Reprogramming of Carbon Metabolism by the Transcriptional Activators Acuk and Acum in Aspergillus Nidulans. Mol Microbiol 84 (5), 942-964
  22. Wong, K. H., and Struhl, K. (2011) The Cyc8-Tup1 Complex Inhibits Transcription Primarily by Masking the Activation Domain of the Recruiting Protein. Genes Dev 25 (23), 2525-2539
    Featured in: Parnell, E.J., and Stillman, D.J. (2011) Shields up: the Tup1−Cyc8 repressor complex blocks coactivator recruitment.” Genes and Development 25, 2429-2435
  23. Tirosh, I.*, Wong, K. H.*, Barkai, N., and Struhl, K. (2011) Extensive Divergence of Yeast Stress Responses through Transitions between Induced and Constitutive Activation. Proc Natl Acad Sci USA 108 (40), 16693-16698 (* Equal contribution)
  24. Wong, K. H., Hynes, M. J., Todd, R. B., and Davis, M. A. (2009) Deletion and Overexpression of the Aspergillus Nidulans GATA Factor AreB Reveals Unexpected Pleiotropy. Microbiology+ 155 (Pt 12), 3868-3880
  25. Wong, K. H., Hynes, M. J., and Davis, M. A. (2008) Recent Advances in Nitrogen Regulation: A Comparison between Saccharomyces Cerevisiae and Filamentous Fungi. Eukaryot Cell 7 (6), 917-925
  26. Wong, K. H., Todd, R. B., Oakley, B. R., Oakley, C. E., Hynes, M. J., and Davis, M. A. (2008) Sumoylation in Aspergillus Nidulans: sumO Inactivation, Overexpression and Live-Cell Imaging. Fungal Genet Biol 45 (5), 728-737
  27. Wong, K. H., Hynes, M. J., Todd, R. B., and Davis, M. A. (2007) Transcriptional Control of nmrA by the bZIP Transcription Factor MeaB Reveals a New Level of Nitrogen Regulation in Aspergillus Nidulans. Mol Microbiol 66 (2), 534-551

Book Chapter

  • Davis, M.A. and Wong, K.H. “Chapter 23: Nitrogen Metabolism in Filamentous Fungi.” In: Cellular and Molecular Biology of Filamentous Fungi edited by Borkovich, Katherine A. and Ebbole, Daniel J. © 2010 American Society for Microbiology (ASM). (Invited book chapter)
Awards
2013Marine Biological Laboratory Scholarship, Marine Biological Laboratory, USA
2009-2010Croucher Postdoctoral Fellowship,The Croucher Foundation, Hong Kong
2003-2006International Postgraduate Research Scholarship (IPRS), The Australian Government
2003-2006Melbourne International Research Scholarships (MIRS), The University of Melbourne, Australia
2001-2003Howitt Natural History Scholarship, The University of Melbourne, Australia
2003The Department of Genetics Postgraduate Scholarship, The University of Melbourne, Australia
2002The Department of Genetics Honours Scholarship, The University of Melbourne, Australia
2002Dean’s Honour List – B.Sc.(Hons), Faculty of Science, The University of Melbourne, Australia
Professional Activities
  • Member of the Genetics Society of America